Biologists continuous studies and research lead to the discovery of stem cells.

While human rights activists lobby for the halting of such procedures, successful operations have indeed proven the effectiveness of stem cell developments in organ transplants.

Thus, it is vital for students or any individuals interested in the field to differentiate between RT-PCR and qPCR.

This laboratory technique is widely used in molecular biology in order for the scientists to produce multiple copies of a particular DNA sequence through a process coined as amplification.

The difference between RT-PCR and traditional PCR is that RNA is first transcribed in reverse into its DNA complement, which utilizes the reverse transcriptase.

To avoid confusion, biologists label the real-time PCR as quantitative real-time PCR or qPCR.

It can be said that RT-PCR starts the amplification process, while qPCR measures it as the procedure takes place.

It is currently involved in quantitative gene expression analysis, genotyping, pathogen detection, and analysis of SNP, along with RNA interference measurements.

This then gives another visible difference between the two: RT-PCR can be used for the amplification process, but it needs to be fused with qPCR for quantification purposes.

Biologists note that the quantity of the byproduct of PCR is directly proportional to the amount of template nucleic acid that will be measured through qPCR.

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Semi Quantitative Pcr